The production of these products in large quantities leads to enormous quantities of whey as a byproduct in the dairy industries, which represents 85-95% of the milk volume and retains 55% of milk nutrients. Among the most abundant of these nutrients are lactose, soluble proteins, lipids and mineral salts (Marwaha and Kennedy, 1988; Mawson, 1994; Gonzalez-Siso, 1996). The availability of carbohydrate reservoir of lactose in whey and presence of other essential nutrients for the growth of microorganisms makes the whey one of the potential substrate for the production of different bio-products through biotechnological means. The production of lactic acid production through lactic acid bacteria could be an alternative processing route for whey lactose utilization.