Isolation of actinomycetes from soil sample Each sample was divided in to two parts One part was used as wet sample and the second part was placed in dryer for one week to be used as dry sample. The samples were serially diluted up to the 10-3 dilution. Aliquot of 0.2 ml of each dilution was inoculated in duplicate on to the plates ofthe ISP2 medium by the spread plate technique (Shirling and Gottlieb, 1966). All the inoculated plates were incubated at 37°C for 7 days. By the pure culture technique strains of actinomycetes were isolated using medium incorporated with 0.5 mg nystatin and nalidixic acid as antifungal and antimicrobial agent respectively