Suspensions of micro-organisms were prepared and
turbidity of each suspension was equal to turbidity of
McFarland No. 0.5. Muller Hilton agar base plate (for
antibacterial test) and Sabouround agar base plate (for
antifungal test) were mixed with suspension of microorganism
and each plate consisted of 6 cups. Diameter
of each cup was 8.0 mm. Hexane, dichloromethane and
methanol crude extracts were dropped into the cups.
Agar plates were incubated under optimum condition for
each micro-organism