production level was obtained [7], the major fatty acids of the
accumulated lipids were C18:0 (stearic acid) and C18:1 (oleic
acid). The results obtained in this work indicate that crude
glycerol is a potential good carbon source for algal DHA
production. In the following sections, the algal culture
conditions including crude glycerol concentration, other
medium nutrients, pH and temperature were optimized to
achieve a high level of cell growth and DHA production from
crude glycerol.