Many factors such as genotype, physiological age of donor plant and culture medium are effective
in anther culture reaction for androgenic callus and plant regeneration. Culture medium and genotype are important factors for callus induction from anther culture[9]. Though different protocols have been proposed to improve the efficiency of the anther culture,the green plant regeneration frequencies still remained rather low, especially for indica (O. sativa ssp. indica) cultivars[10]. The aim of this study was to evaluate the culture efficiency of anthers derived from F1 to several hybrid generations by different culture media, and establish the reliable system for high green plantlet. It was of great significance to improve rice cultivars and enrich, the genetic background in rice breeding.