2.8. Statistical analyses
All the experiments were repeated and the presented data are average of the two experiments.
For each analysis, there were five replicated (independent) tissue samples. The data for germination studies were subjected to dose–response analysis.
Dose–response curves were derived by plotting the concentration (on the x-axis) and percent response (on the y-axis) using GraphPad Prism version 5, and LC50 values determined from the dose–response curve.
The effect of essential oil on shoot and root length, chlorophyll
content, respiratory value, visible injury, and relative electrolyte
leakage was analyzed by linear regression models.