There are no apoptotic
changes in the nuclei of cells which are identified as
dead. Both propidium iodide and Hoechst dye stain the
nucleus, which gives purple-like color. There is no significant
change in the nuclear pattern. But the nucleus
of a necrotic cell may be observed larger than normal.
The intensity of the dye may be normal at the beginning,
but it may be weaker in later stages due to the lysis of
the cell.