Three lactic acid bacteria (LAB) designated as FB2, FF2 and FG1
used in this study were isolated from barley, fermented olive and
traditional dried meat respectively. Briefly, ten grams of each
sample were homogenized in 90 mL of Man Rogosa and Sharpe
(MRS) broth and incubated at 37 C for 48 h. Then, 0.1 mL of the
culturewas spread on MRS agar and incubated for 48 h at 37 C. The
obtained colony was selected using positive Gramstain, production
of catalase and cytochrome oxidase. The biochemical characterization
of the isolates was determined using the API 50 CHL (Bio-
Merieux, France). The potential probiotic strains were conserved at
80 C in MRS broth with 30% glycerol.
Three lactic acid bacteria (LAB) designated as FB2, FF2 and FG1used in this study were isolated from barley, fermented olive andtraditional dried meat respectively. Briefly, ten grams of eachsample were homogenized in 90 mL of Man Rogosa and Sharpe(MRS) broth and incubated at 37 C for 48 h. Then, 0.1 mL of theculturewas spread on MRS agar and incubated for 48 h at 37 C. Theobtained colony was selected using positive Gramstain, productionof catalase and cytochrome oxidase. The biochemical characterizationof the isolates was determined using the API 50 CHL (Bio-Merieux, France). The potential probiotic strains were conserved at80 C in MRS broth with 30% glycerol.
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