The objective of this study was to investigate the in vitro free radical scavenging
activity and protective effects of soymilk fermented by Lactobacillus fermentum
Zhao (FSM-FLZ) in hydrogen peroxide (H2O2)-induced porcine renal proximal
tubule LLC-PK1 cells. FSM-FLZ was effective against DPPH and •OH radical in a concentration-dependent manner. FSM-FLZ treatment significantly increased cell viability and reduced the H2O2-induced oxidative injury in LLC-PK1 cells. In
addition, FSM-FLZ dose-dependently decreased the intracellular reactive oxygenspecies (ROS) levels and lipid peroxidation, and increased the activities and mRNA expressions of endogenous catalase and superoxide dismutase enzymes.
This study demonstrated that FSM-FLZ is a free radical scavenger and protects
against H2O2-induced oxidative injury in LLC-PK1 cells through reducing intracellular
ROS levels, inhibiting lipid peroxidation and increasing the antioxidant
enzyme activity.