Folin-Ciocalteu assay was used to estimate TPC (Saxena et al.,
2010). A 25 ml of a 100 g/l honey solution was added to each well
of a 96 well micro plate followed by 50 ml Folin-Ciocalteu and 50 ml
of a 75 g/l Na2CO3 solution, thoroughly mixed and the absorbance
was read at 765 nm.