principle of these structures is based on the use of capillary force
to wick and distribute fluid into hydrophilic channels without
external pressure or control systems. Three-dimensional devices
of these types have been fabricated by stacking layers of
patterned paper and tape,18 or by sewing threads into hydrophobic
substrates.20 These methods, however, still have some
inherent difficulties to be used for mass production of the devices,
as well as for determining the exact detection zones in the case of
thread-based devices.20 The Whitesides group has proposed the
use of the wax printing technique as a cheaper way of mass
producing mPADs.22 Yet, this technique requires the use of
a special type of wax printer that even though is commercially
available, may not be easily found in many of the developing
countries. Most recently, silk was used by Dendukuri et al. to
define a fabric-based platform for diagnostic assays.23 However,
silk cloth is usually expensive to be used as a disposable substrate
for microfluidic devices.
principle of these structures is based on the use of capillary forceto wick and distribute fluid into hydrophilic channels withoutexternal pressure or control systems. Three-dimensional devicesof these types have been fabricated by stacking layers ofpatterned paper and tape,18 or by sewing threads into hydrophobicsubstrates.20 These methods, however, still have someinherent difficulties to be used for mass production of the devices,as well as for determining the exact detection zones in the case ofthread-based devices.20 The Whitesides group has proposed theuse of the wax printing technique as a cheaper way of massproducing mPADs.22 Yet, this technique requires the use ofa special type of wax printer that even though is commerciallyavailable, may not be easily found in many of the developingcountries. Most recently, silk was used by Dendukuri et al. todefine a fabric-based platform for diagnostic assays.23 However,silk cloth is usually expensive to be used as a disposable substratefor microfluidic devices.
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