Outbreaks of verotoxigenic Escherichia coli (VTEC) linked to dry-fermented sausages (DFSs) have
emphasized the need for DFS manufacturers to introduce measures to obtain enhanced safety and still
maintain the sensory qualities of their products. To our knowledge no data have yet been reported on non-
O157:H7 VTEC survival in DFS. Here, the importance of recipe and process variables on VTEC (O157:H7 and
O103:H25) reductions in two types of DFS, morr and salami, was determined through three statistically
designed experiments. Linear regression and ANOVA analyses showed that no single variable had a dominant
effect on VTEC reductions. High levels of NaCl, NaNO2, glucose (low pH) and fermentation temperature gave
enhanced VTEC reduction, while high fat and large casing diameter (aw) gave the opposite effect. Interaction
effects were small. The process and recipe variables showed similar effects in morr and salami. In general,
recipes combining high batter levels of salt (NaCl and NaNO2) and glucose along with high fermentation
temperature that gave DFS with low final pH and aw, provided approximately 3 log10 reductions compared to
approximately 1.5 log10 reductions obtained for standard recipe DFS. Storage at 4 °C for 2 months provided
log10 0.33–0.95 additional VTEC reductions and were only marginally affected by recipe type. Sensory tests
revealed only small differences between the various recipes of morr and salami. By optimisation of recipe
and process parameters, it is possible to obtain increased microbial safety of DFS while maintaining the
sensory qualities of the sausages.
Outbreaks of verotoxigenic Escherichia coli (VTEC) linked to dry-fermented sausages (DFSs) have
emphasized the need for DFS manufacturers to introduce measures to obtain enhanced safety and still
maintain the sensory qualities of their products. To our knowledge no data have yet been reported on non-
O157:H7 VTEC survival in DFS. Here, the importance of recipe and process variables on VTEC (O157:H7 and
O103:H25) reductions in two types of DFS, morr and salami, was determined through three statistically
designed experiments. Linear regression and ANOVA analyses showed that no single variable had a dominant
effect on VTEC reductions. High levels of NaCl, NaNO2, glucose (low pH) and fermentation temperature gave
enhanced VTEC reduction, while high fat and large casing diameter (aw) gave the opposite effect. Interaction
effects were small. The process and recipe variables showed similar effects in morr and salami. In general,
recipes combining high batter levels of salt (NaCl and NaNO2) and glucose along with high fermentation
temperature that gave DFS with low final pH and aw, provided approximately 3 log10 reductions compared to
approximately 1.5 log10 reductions obtained for standard recipe DFS. Storage at 4 °C for 2 months provided
log10 0.33–0.95 additional VTEC reductions and were only marginally affected by recipe type. Sensory tests
revealed only small differences between the various recipes of morr and salami. By optimisation of recipe
and process parameters, it is possible to obtain increased microbial safety of DFS while maintaining the
sensory qualities of the sausages.
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