Analysis of physicochemical properties of proteins showed that
CPI is more susceptible to thermal denaturation than GCPI during
heat treatment as evidenced by higher denaturation degree and
higher loss of solubility. This is attributed to the protective effect
of higher glycation degree and higher carbohydrate content of GCPI
as demonstrated by sugar analysis and glycoprotein staining of SDS
PAGE gels, resulting in improved maintenance of solubility. Water
absorption of bread dough was significantly increased by DCPI and
to a larger GCPI compared to the control, resulting in softer texture.
Sensory testing and textural analysis of bread indicate that denaturation
and glycation of CPI can allow the inclusion level in bread
to be increased from 2% to 4% without affecting the sensory acceptability
or textural properties.