Methylotrophs play a major role for saving our environments by utilizing toxic methanol, which are hazardous to environment as well as to human being hence attempt was made to degrade it by eco-friendly microbial techniques which is cost effective, cheap and free from ill effect. In present study, two bacterial strains Ps. hibiscicola (DHT 11) and Ps. aeruginosa (DHT 12) were isolated from sediment samples by using enrichment technique, in which minimal salt medium containing 2% methanol used as sole source of carbon and energy. The bacterial strains were characterised by cultural, morphological, biochemically and 16S rRNA gene sequencing. The selected microbial strain was able to utilized methanol up to 82% by Ps. hibiscicola and 79%
by Ps. aeruginosa. Therefore these two bacterial cultures can be employed effectively to bioremediation of methanol and other C1 compounds on polluted sites. Outcome of this study may offer useful information in evaluating potential bacterial methanol degraders from the environment.