factor of 6.25. Total residual sugar was determined by using a Megazyme
total starch kit (Megazyme International Ltd., Wicklow,
Ireland) following the procedure of Srichuwong and Jane (2011).
Ash content was determined by heating the dry stillage at 550 C
for 5 h (AOAC 923.03). Total carbohydrate content was obtained
by subtracting the oil, protein, and ash contents from 100%. Total fiber
content was obtained by subtracting the total residual sugar
content from total carbohydrate content. Amino acid content was
determined by the University of Missouri Agricultural Experiment
Station Chemical Laboratories (Columbia, MO).
2.10. Total