Brine shrimp cytotoxicity
Brine shrimps’ eggs were hatched in laboratory at room temperature. 10 shrimps, 5 ml sea water and extract in concentrations 10, 100 and 1000 ppm were added to a vial. An incubation period of 24 hours was given at room temperature. Visual counting of dead brine shrimps (laying at bottom) was performed. Per cent cytotoxicity was determined