The column was activated to obtain a better separation results.
Separation of catechins with the stationary phase in column
chromatography was done by polyamide with 10% ethanol
eluent and 95% ethanol. Column chromatography separation
with a polyamide stationary phase can separate analyzed compound
based on the polarity. About 10% ethanol-water elution
was used for separating catechins with a fairly high polarity.