Characterization of extracted proteins in natural rubber latex
Proteins from natural rubber latex were distributed into two fractions, the rubber and serum fractions. To characterize these proteins they were extracted after processing using several chemical reactions and washing with surfactant solution. Removal of protein from rubber particles was confirmed by monitoring the decrease in protein content of treated latex. The proteins removed from latex were analyzed using SDS–PAGE technique, as shown in Fig. 1. SDS–PAGE gel image presents the standard proteins (lane A), soluble proteins (lane B), decomposed proteins after deproteinization reaction (lane C), decomposed proteins after saponification reaction (lane D), SDS-extracted proteins from large rubber particles (lane E) and SDS-extracted proteins from small rubber particles (lane F).
Characterization of extracted proteins in natural rubber latexProteins from natural rubber latex were distributed into two fractions, the rubber and serum fractions. To characterize these proteins they were extracted after processing using several chemical reactions and washing with surfactant solution. Removal of protein from rubber particles was confirmed by monitoring the decrease in protein content of treated latex. The proteins removed from latex were analyzed using SDS–PAGE technique, as shown in Fig. 1. SDS–PAGE gel image presents the standard proteins (lane A), soluble proteins (lane B), decomposed proteins after deproteinization reaction (lane C), decomposed proteins after saponification reaction (lane D), SDS-extracted proteins from large rubber particles (lane E) and SDS-extracted proteins from small rubber particles (lane F).
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