The lysates were centrifuged and supernatants were removed. The microtubes were then placed in a microwave oven (with
specifications; LG grill, model No. MG-604AZ, input 220v-50/bHz,
microwave 1350 w, RF output 900 w,2,4500 MH) and heated twice
for 1 min at 900 W or three times for 1 min at 750 W. The pellets
were dissolved in 200 µl of TE and were extracted with an equal
volume of phenol/chloroform/ isoamyl alcohol (25:24:1) for 15 min.
The aqueous phase was recovered by centrifugation for 20 min and
precipitated with ethanol
The lysates were centrifuged and supernatants were removed. The microtubes were then placed in a microwave oven (withspecifications; LG grill, model No. MG-604AZ, input 220v-50/bHz,microwave 1350 w, RF output 900 w,2,4500 MH) and heated twicefor 1 min at 900 W or three times for 1 min at 750 W. The pelletswere dissolved in 200 µl of TE and were extracted with an equalvolume of phenol/chloroform/ isoamyl alcohol (25:24:1) for 15 min.The aqueous phase was recovered by centrifugation for 20 min andprecipitated with ethanol
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