The Troll software identified 7,270 SSRs which represent an average frequency of EST-SSR of one SSR in every 2,424 bp in the cassava transcriptome. The frequency of EST-SSR found in cassava was similar to that found in Hevea brasiliensis(2.24 kb) . Both cassava and rubber tree are closely related in the same Euphorbiaceae family where a large proportion of EST markers .