owadays there is general consensus that climate change is mainly caused by anthropogenic activities due to the use of fossil fuels as primary energy source. In OECD countries the transport sector is responsible for at least 23% of global CO2 emissions with an increasing rate [1]. Global CO2 emissions reached 34 billion tons in 2011, a value 3% higher than the previous year, and higher than the annual average increase of 2.7% in the last decade [2]. The European Environment Agency (EEA) established a 5.2% reduction in CO2 emissions for 2012, considering as a base the emissions in 1990, to meet the agreements established in the Kyoto Protocol, though which is expected that biofuels will provide 27% of the total transport fuel by 2050 [3]. However, a report from the Bioethanol for Sustainable Transport Project (BEST) suggests that additional agreements are needed to meet the goal of the European Commission of 20% emissions reduction by 2020 [4]. Actions like blending mandates, tax incentives, and purchasing policies have been essential for the development of the production of biofuels in Europe, Asia, United States of America, Brazil and other countries. In USA a framework for biofuels have been implemented, it includes the U.S. Renewable Fuels Standard (RFS) and the Energy Independence and Security Act of 2007 [5] aiming to promote the use of biofuels in the transport sector. In the European Union the European Renewable Energy Directive was announced in 2009 [6].