7. Methods for developing yeast strains suitable for SSF
7.1. Site-directed mutagenesis
Using site-directed mutagenesis (SDM), also known as oligonucleotide-directed mutagenesis or site-specific mutagenesis, specific desired changes to the DNA sequence of a particular gene can be effected, resulting in altered gene products. SDM is key to investigating the structure and biological function of DNA, RNA, and proteins and also to protein engineering.
A DNA primer with the desired