Western blot analysis: bacterial lysates of B. longum expressing GLBP-flagellin
protein. Lane M, protein molecular markers (kDa); Lane P.C., positive control (10 ng
of flagellin derived from S. Typhimurium); Lane Pa, 5g protein lysates of parental
B. longum; Lane R, 5g protein lysates of the recombinant B. longum. In lane R, the
band corresponded to a weight of approximately 98 kDa, which is the total molecular
weight of flagellin plus GL-BP, was confirmed