No change in inhibitory activity against L. innocua was recorded when the CFS was treated at pH settings between 2.0 and 10.0. Heating progressively reduced the inhibitory activity of the supernatant; the inhibition was completely lost after treatment at 121 °C for 15 min. As a consequence it cannot be used in foods submitted to thermal sterilization.
The results of bacteriocin stability throughout the storage time showed that the maximum inhibitory activity remained constant up to 6 months when the supernatant was stored at 4 °C, but after 6 months of storage there was a decrease of 50% in the activity. In contrast, 100% of the initial activity was observed after 1 year storage at −30 °C. Therefore, the bacteriocin can be applied to foods preserve by freezing.