2.2.2. Physical sexual development
The anogenital distance (AGD, distance from the anus to the genital
tubercle) was obtained weekly (PND 21, 28, 35, 42) through a vernier
caliper until occurrence of preputial separation. AGD was normalized
through its division by the cube root of body weight. From PND 45 [25], preputial separation was verified daily and considered as an
indicator of the sexual maturity onset.