Briefly, the host for
recombinant DNA manipulations was Escherichia coli
strain NovaBlue (Novagen, Madison, WI, USA), and
cellulolytic enzymes were expressed in the haploid yeast
strains S. cerevisiae MT8-1 [17] and NBRC1440ΔHUWL
[13]. The haploid and diploid S. cerevisiae strains 1440/
cocδBEC3 and MNII/cocδBEC3, respectively, were constructed
as described below.
E. coli transformants were
Briefly, the host forrecombinant DNA manipulations was Escherichia colistrain NovaBlue (Novagen, Madison, WI, USA), andcellulolytic enzymes were expressed in the haploid yeaststrains S. cerevisiae MT8-1 [17] and NBRC1440ΔHUWL[13]. The haploid and diploid S. cerevisiae strains 1440/cocδBEC3 and MNII/cocδBEC3, respectively, were constructedas described below.E. coli transformants were
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