The sensitivity of both MS-H1 and MS-H2 melt-MAMAs was 103 copy numbers, while
both the MS-H1 and MS-H2 agarose-MAMAs showed a sensitivity of 104 copy numbers. Neither assay cross-reacted with the other tested avian Mycoplasma species. The M. synoviae type
strain (WVU 1835, NCTC 10124), the seven clinical M. synoviae isolates and 35 out of 40 trachea swabs taken from clinically infected chickens showed the wild-type strain profile while
the ts+ MS-H vaccine strain (Vaxsafe MS-H), the nine ts+ MS-H re-isolates and 38 out of 40
trachea swabs taken from vaccinated chickens showed a ts+ MS-H vaccine strain profile in the
melt and agarose-MAMAs. These results were confirmed by sequencing of the obg gene of
these samples as well (data not shown).