Red muscadine grapes (cv. Novle) for juice and wine preparation were obtained from a local grower in Central Florida and transported to the Food Science and Humun Nutrition department, FL for immediate processing into grape juice. Grapes (40kg) were crushed, miwed with 20 mL biopectinase (Quest International, Hoffman Estates, Ill), gradually heated to C, and held for 30 miun. Grapes were pressed into juice (ca. 70% yield) using a hydraulic basket press at 2500 psi (Prospero’s Equipment, Cort, NY) and stored frozen at C until needed. Juice was successively filtered through cheesecloth, What man #4 filter paper, and finally a 0.45 µm membrane filter for physicochemical evaluations.