Enzymatic hydrolysis was carried out using xylanase, to remove the hemicelluloses and lignin fractions. The light yellowish pulp became lighter, but not totally white (Fig. lb), so the process did not remove the lignin fractions completely. Probably, the alkaline treatment probably was not efficient enough to totally remove lignin; the remaining lignin may have formed a barrier around hemicelluloses and cellulose. In other words, the enzyme xylanase had difficulty in solubilizing hemicelluloses, so it was not able to penetrate the cellulose chains, which made cellulose hydrolysis difficult. Compared with ET, CT involved more steps and was more aggressive, promoting greater removal of the amorphous components.
The concentration of nanofibers in the suspensions (g of nanofibers/100 g of suspension) obtained from CT and ET was 0.54 and 0.57, respectively. Regarding nanoparticles yield, ET furnished twice as high yield (10%) as CT (5.1%). The extra steps performed during CT probably culminated in greater sample loss.
Enzymatic hydrolysis was carried out using xylanase, to remove the hemicelluloses and lignin fractions. The light yellowish pulp became lighter, but not totally white (Fig. lb), so the process did not remove the lignin fractions completely. Probably, the alkaline treatment probably was not efficient enough to totally remove lignin; the remaining lignin may have formed a barrier around hemicelluloses and cellulose. In other words, the enzyme xylanase had difficulty in solubilizing hemicelluloses, so it was not able to penetrate the cellulose chains, which made cellulose hydrolysis difficult. Compared with ET, CT involved more steps and was more aggressive, promoting greater removal of the amorphous components.The concentration of nanofibers in the suspensions (g of nanofibers/100 g of suspension) obtained from CT and ET was 0.54 and 0.57, respectively. Regarding nanoparticles yield, ET furnished twice as high yield (10%) as CT (5.1%). The extra steps performed during CT probably culminated in greater sample loss.
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