Finally after increasing the temperature to 230 ◦C with 10 ◦C/min it remained for 10 min.
The temperatures of the injector and detector were set at 250 ◦C and 300 ◦C, respectively.
N,N-dimethylanilin was used as an internal standard and methyl esters content was calculated by calibration curve using
area peak.
The gas chromatogram of the methyl esters in the product sample is shown in Fig. 3. The yield of the biodiesel production
was calculated by the following equation