The data for analyzing quantitatively the contributions of the two earthworm effects, earthworm feeding and
earthworm–microorganism interaction, to the enhanced VSS reduction were outlined in Table 2, and the results were shown in Table 3. The results based on 15N showed that the earthworm feeding and earthworm microorganism interaction were responsible for approximately 21% and 79%, respectively, of the enhanced VSS reduction. However, the results according to the earthworm biomass and yield rate of earthworm cast were distinct, and showed the two effects were responsible for approximately 40% and 60%, respectively. In the previous study by Zhao et al. [2], the method to estimate the amount of earthworm cast was to select
some earthworms randomly, and to culture the earthworm in a dish in the dark for 24 h, and then to determine the excrement in the culture dish, and finally to estimate the yield rate of earthworm cast (g SS kg−1 earthworm d−1), combining with the earthworm biomass in the whole VF. However, the results according to
the method might be the presence of large deviation as the considerable difference in the environments between the dish and VF system might lead to a significant variation in the yield rate of earthworm cast. Additionally, it was difficult to estimate exactly the earthworm biomass in the whole VF, especially in the full-scale system. Thus, the methods according to the earthworm biomass and the yield rate of earthworm cast were less feasible and accurate than that based on 15N.
The data for analyzing quantitatively the contributions of the two earthworm effects, earthworm feeding andearthworm–microorganism interaction, to the enhanced VSS reduction were outlined in Table 2, and the results were shown in Table 3. The results based on 15N showed that the earthworm feeding and earthworm microorganism interaction were responsible for approximately 21% and 79%, respectively, of the enhanced VSS reduction. However, the results according to the earthworm biomass and yield rate of earthworm cast were distinct, and showed the two effects were responsible for approximately 40% and 60%, respectively. In the previous study by Zhao et al. [2], the method to estimate the amount of earthworm cast was to selectsome earthworms randomly, and to culture the earthworm in a dish in the dark for 24 h, and then to determine the excrement in the culture dish, and finally to estimate the yield rate of earthworm cast (g SS kg−1 earthworm d−1), combining with the earthworm biomass in the whole VF. However, the results according tothe method might be the presence of large deviation as the considerable difference in the environments between the dish and VF system might lead to a significant variation in the yield rate of earthworm cast. Additionally, it was difficult to estimate exactly the earthworm biomass in the whole VF, especially in the full-scale system. Thus, the methods according to the earthworm biomass and the yield rate of earthworm cast were less feasible and accurate than that based on 15N.
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