Switch on the electrical source and high voltage brake and then adjust the high-voltage pulsed generator’s parameters as follows: output voltage 30 kv, discharging frequency 50 Hz, and action time180 s. Then press the ignition switch to generate high voltage pulse in the treatment chamber. The timing-relay switches cuts off the electrical source when it is the preset time. This will lead to the cease of the system. Take out the sample in the treatment chamber also using a sterile injector after the voltage drops to 0 V.