Chandra et al.degraded Kraft lignin by three bacterial isolates which were able to reduce 69% color, 40% lignin and total substrate by 50% after 48 h. On the other hand, Chandra et al. used Bacillus cereus (ITRC-S6) and Serratia marcescens for reduceding color, lignin , BOD , COD, in 7-day period supplementing the sample with glucose and peptone