To evaluate the survival of L. rhamnosus GG during encapsulation,
cell counts were determined before and after spray-drying.
Cell counts were obtained by determining the number of CFU in
1 mL concentrate before spray-drying and in 1 g spray-dried
powder. For this purpose, 1 mL (concentrate) or 1 g (powder)
sample was introduced in 9 mL of tryptone salt broth and the solutionwas
mixed during 2 min using a vortex homogenizer. Sample
was serially diluted in tryptone salt broth and plated on MRS agar.
After 48 h incubation at 37 C, cell counts were determined and
expressed as CFU g1. The concentrate density was measured to be
able to translate the 1mL of concentrate in grams. For this, a known
volume of concentrate was weighed. The experiment was realized in triplicate for each formulation.