To
this end, a 10 mL aliquot of the methanol extract was mixed in a 1:2
ratiowith 0.4Mammoniumsulfate and the solutionwas left on a shaker
for at least 20 h. The resulting precipitate containing the non-saponin
constituents was removed by centrifugation (3.225 ×g, 30 min) at
roomtemperature