The amount of rutin deposited in the epidermal and the dermal layers (excluding the stratum corneum) increased in the following order: ethosome(9.30%; 8.68±0.03 µg/cm2)>liposome (6.78%; 6.33±1.34 µg/cm2)>ethanolic solution (6.57%; 6.13±0.14µg/cm2)>DW solution (4.05%;3.78±0.13 µg/cm2).