Flavonoid estimation Aluminum chloride colorimetric method was used for flavonoids determination (Chang et al., 2002). 0.5 mL of each plant extract was separately mixed with 1.5 mL of methanol, 0.1 mL of 10% aluminum chloride, 0.1 mL of 1 M potassium acetate and 2.8 mL of distilled water. The reaction mixture was allowed to stand at room temperature for 30