3.3. Concentration comparison (Table 1 and Table 2)
Freeze drying method - higher concentration of colchicine was extracted from tuber sample and found to be 0.44 mg/ml.
Dichloromethane method - higher concentration of colchicine was extracted from tuber sample and found to be 0.74 mg/ml.
Hot water method - higher concentration of colchicine was extracted from leaf sample and found to be 1.42 mg/ml.
Sonication method - higher concentration of colchicine was extracted from pod sample and found to be 0.21 mg/ml.
Among the above four methods of extraction, higher concentration of colchicine was extracted in Dichloromethane
method and very lower concentration of colchicine was extracted in Sonication method.Also, Hot water method was found to
be the cheapest method of colchicine extraction as water was the only solvent used for extraction. Tuber was found to be
having higher amount of colchicine in solvent extraction but in hot water method, water cannot extract colchicine from tuber,
so increasing the temperature and pressure can extract higher amount of colchicine from tuber samples.