We further examined the optimal initial CSL concentration
in agitated culture conditions. In tests in a fermentor,
the maximum cellulose yield was obtained in
8% CSL medium, different from the results shown in
Fig. 4. It was, therefore, preferential to perform agitated
cultures in 8% CSL medium. As shown in Fig. 5 and
Table 2, the fermentation process, which involved cellulose
production by A. xylinum BRCS, aerobically cultivated
in 8% CSL medium containing 2% glucose, comprised
the following: a) Lag phase or induction phase
(O-22 h) in which cells did not grow, but substantial
amount of gluconic acid accumulated almost proportionally
to the same amount of glucose being consumed. b)
Accumulation phase of gluconic acid (22-30 h) in which
almost all glucose was rapidly metabolized to gluconic
acid and the cells began to grow exponentially. c) Cell
growth/cellulose production phase (30-43 h) in which the
cells continued to grow and cellulose began to be
produced rapidly with decreasing amounts of accumulated
gluconic acid. d) Stationary phase (43-49 h) in which
both cell growth and cellulose production ceased even
though considerable amount of gluconic acid still remained.
We further examined the optimal initial CSL concentrationin agitated culture conditions. In tests in a fermentor,the maximum cellulose yield was obtained in8% CSL medium, different from the results shown inFig. 4. It was, therefore, preferential to perform agitatedcultures in 8% CSL medium. As shown in Fig. 5 andTable 2, the fermentation process, which involved celluloseproduction by A. xylinum BRCS, aerobically cultivatedin 8% CSL medium containing 2% glucose, comprisedthe following: a) Lag phase or induction phase(O-22 h) in which cells did not grow, but substantialamount of gluconic acid accumulated almost proportionallyto the same amount of glucose being consumed. b)Accumulation phase of gluconic acid (22-30 h) in whichalmost all glucose was rapidly metabolized to gluconicacid and the cells began to grow exponentially. c) Cellgrowth/cellulose production phase (30-43 h) in which thecells continued to grow and cellulose began to beproduced rapidly with decreasing amounts of accumulatedgluconic acid. d) Stationary phase (43-49 h) in whichboth cell growth and cellulose production ceased eventhough considerable amount of gluconic acid still remained.
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