The virulence of this strain in tomato was assessed in greenhouse
tests by stem-inoculating tomato plants (cv. Money-Maker)
at the 4-leaf stage with 30 ml of a bacterial suspension containing
108 cfu ml1 (Zanón and Jordá, 2008). After inoculation, plants were
maintained under greenhouse conditions at 18e30 C. Symptoms
of bacterial wilt were assessed 15 days after inoculation. The
presence of the bacterium was confirmed using a Double Antibody
Sandwich-Enzyme Linked Immunosorbent Assay (DAS-ELISA) with
the Loewe commercial antiserum No. 07056 (Loewe Biochemica
GmbH, Sauerlach, Germany).