The two stage enzymatic hydrolysis treatment was carried out in the pretreated fruit samples with the aim of firstliquefying the starch at higher temperature (90ºC) by amylase and then the second stage was performed at a lowtemperature (60ºC) by glucoamylase to convert the starch completely to monomeric sugars. The liquefaction of starch by α-amylase from Bacillus subtilis under high temperatures (90 110ºC) and saccharification of the liquefied starchby glucoamylase fromor Aspergilus niger Rhizopus sp. at low temperatures (60 70ºC) have been extensively studiedby many authors [43,44]