The plate was incubated at 37 ˚C for 30 min in Microplate Reader. Then, 25 mL of freshly prepared 2, 20 Azobis (2-methylpropionamidine) dihydrochloride (153 mmol/L) was added to all the designated wells
The plate was incubated at 37 ˚C for 30 min in Microplate Reader. Then, 25 mL of freshly prepared 2, 20 Azobis (2-methylpropionamidine) dihydrochloride (153 mmol/L) was added to all the designated wells