Thermal manipulation during incubation has been shown to improve post hatch performance
in poultry. The aim of the present experiment was to evaluate thermal manipulation
on semen quality of roosters during hot climatic conditions. Eggs obtained after arti-
ficial insemination from Dahlem Red layer breeders were randomly divided into two
groups control (C) and heat exposed (HE). C group eggs were incubated at 37.5 ◦C
throughout the incubation period while the HE group eggs were exposed to higher temperature
40.5 ◦C from 15th to 17th day of incubation for 3 h each day. The relative
humidity was maintained at 65% in both the groups throughout incubation. The chicks
hatched were reared separately under standard husbandry conditions. During high ambient
temperature semen from roosters (45 weeks of age) was collected and evaluated
for different gross parameters, sperm chromatin integrity and sperm HSP27 and HSP70
gene expression by real-time PCR. The seminal plasma was evaluated for lipid peroxidation,
ferric ion reducing antioxidant power (FRAP), triiodothyronine (T3) and matrix
metalloproteinase-2 (MMP-2) activity. The shed average Temperature Humidity Index
(THI) during the experiment period was 78.55. The percent live sperm and FRAP level
were significantly (P < 0.05) higher and sperm gene expressions were significantly (P < 0.05)
lower in the HE group. No differences in other parameters were observed between the
groups. Thus from the results it could be concluded that thermal manipulation during
incubation improves certain semen parameters of roosters at high ambient temperature.