DPPH is one of the free radicals widely used for testing preliminary radical scavenging activity of a
compound or a plant extract.
i) Qualitative assay: The color changes (yellow on purple background) on the TLC plates were
observed due to the bleaching of DPPH by the resolved bands.
ii) Quantitative assay: The methanol extract of the aerial part of Mimosa pudica showed
moderate antioxidant activity with the IC50 value of 296.92 μg/ml against DPPH free radical (Table-
3). It is evident that the extract possesses antioxidant activity.