The extraction of flavanones was based on the procedure of Sánchez-Moreno
et al. (2003b). Sample (5 g) was homogenised with
15 ml 80% aqueous methanol and centrifuged. An aliquot of this
extract was used for subsequent flavanone aglycons determination
after acid hydrolysis. To 800 ll methanolic extract, 200 ll of 6 M
HCl was added. Hydrolysis was carried out by refluxing on a water
bath at 90 C for 30 min. After hydrolysis, the sample was cooled
and filtered through a 0.45-lm membrane filter and duplicates
of 50 ll for each extract were analysed by HPLC.