These columns were packed uniformly with the above-mentioned resin. The resin was pretreated with 2 M, mol/dm3, HCl solution to remove impurities and to convert the resin into H+ form. This was followed by passing a solution of 0.1 M CuCl2 to convert the resin into Cu2+ form. Then a 0.05 M NdCl3 solution was fed into the first column at a constant flow rate by a peristaltic pump to form Nd3+ adsorption band. When the Nd3+ ion adsorption band had grown to an appropriate length, the supply of the feed solution was stopped. The Nd3+ and Cu2+ adsorption bands were eluted by an eluent solution containing 0.2 M ammonium malate or 0.15 M ammonium citrate or 0.2 M ammonium glycolate + 0.1 M NH4NO3 + 0.0002NaN2 adjusted to pH 4.6 with NH4OH solution. The adsorption band of Nd3+ was visible, pink, in contrast with the preceding green Cu band. When the Nd3+ adsorption band migration length reached to the desired length, it was eluted out from the last column. The effluent was collected in small fractions that were, thereafter, subjected to the concentration analysis and the isotopic analysis. The temperatures of the columns were kept constant at 25 ± 0.2 °C by circulating the thermostated water through the water jackets surrounding the columns.