There are no comparable data available on the effect of contaminated semen on the superovulatory response of seronegative embryo donors. In the present circumstance,
an average number of CL based on the day of nonsurgical embryo collection or postmortem appears to be similar to the response in noninfected heifers in our herd. Because
superovulatory treatment was carried on before infection (insemination), it is clear that the hormonal balance responsible for follicular growth and ovulation was not
affected by the viral agent. However, it might have a deleterious effect on embryonic viability and development after insemination. This view can be supported by experiments
in which attempts to superovulate persistently infected cows to produce embryos using virus-free semen were undertaken [29,34–36]. Each of these studies resulted in collection of only a single transfer-quality embryo, a few degenerated embryos, and unfertilized oocytes