Chitosan (>90% DDA viscosity 500–2000 cps) was obtained from France Chitin (Marseille, France). Glycerol, used as a plasticizer to improve coating flexibility, was purchased from Fisher Scientific Inc. (Fair Lawn, NJ).
Strains of Colletotrichum gloeosporioides, Phomopsis sp. RP257, Pestalotiopsis sp. and Lasiodiplodia Theobromae ngr 05A were isolated and identified by CIRAD (Montpellier-France).