Oil palm is a commodity that has potential to be transferred to biodiesel. This study is aimed to elucidate genetic diversity of four population based on yield component, using 27 microsatellites. Analysis of banding pattern resulted average polymorphic loci and PIC are 52.78% and 0.51. One, three, one and two markers specific to the group OB, BN, OY and FFB, respectively. Cluster analysis revealed level of genetic diversity and similarity at 0.56 and 0.78-0.98, which is succesfully separated the four population, as well as confirmed by PCoA, indicated that can be used to elucidate specific markers corresponding to populations used.