For testing amplification efficiency of the LCP, a L. crispatus strain (ATCC #33820) was obtained from ATCC (American Type
Culture Collection) and used. The L. crispatus strain was cultured in MRS agar and DNA was extracted. Near 100% PCR efficiency of the LCP was verified by using a standard curve analysis of L. crispatus DNA dilutions (Suppl. Fig. 1).